本站原创
[摘要] 目的 探讨肺炎支原体（MP）快速鉴定培养基在MP感染早期的临床诊断价值。 方法 采用MP快速鉴定培养基对本院2009年1月～2011年12月患呼吸道感染的1 620例患者咽拭子标本进行MP培养检测。 结果 本组患者咽拭子标本MP培养阳性共656例，总阳性率40.49％，其中男性阳性率40.85％，女性阳性率40.13％，男女性别比较差异无统计学意义（P > 0.05）；≤2岁儿童阳性率45.95%，3～14岁阳性率31.41%，差异有统计学意义（P < 0.05）；夏、秋季阳性率与冬季比较，差异均有统计学意义（均P < 0.05）。 结论 MP快速鉴定培养基检测MP感染总检出率较高，结果准确、简便、快速，可作为一般实验室诊断MP感染的首选试验。
[关键词] 肺炎支原体；快速鉴定；培养基
[] C[文章编号] 1673-7210（2012）08（c）-0156-02
Results and analysis of medium for rapid identification of mycoplaa pneumoniae culture
PENG Ping XU Xiaodu WANG Guangbin YE Dan ZHANG Ting WU Bo
The First People′s Hospital of Longquanyi District in Chengdu City, Sichuan Province, Chengdu 610100, China
[Abstract] Objective To investigate the rapid mycoplaa pneumoniae (MP) identification of culture medium in the diagnosis of early infection. Methods 1 620 cases suffered from respiratory tract infection in our hospital from January 2009 to December 2011 were examined with rapid identification of MP medium. Results 656 cases of the throat swab specimens were confirmed MP positive, with a total positive rate of 40.49%; the positive rate was 40.80% in male and 40.13% in female, the difference between male and female was not statistically significant (P > 0.05); the positive rate was 45.95% of ≤ 2 year children and 31.41% of 3-14 year old children, the difference was statistically significant (P > 0.05); the positive rate in the season of summer and autumn was lower than that in spring, the difference was all statistically significant (all P > 0.05). Conclusion The rapid identification of medium detection of mycoplaa pneumoniae infection has a higher overall detection rate and a accurate, simple, rapid result, it is a laboratory diagnostic test of choice for MP infection.
[Key words] Mycoplaa pneumoniae; Rapid identification; Medium
肺炎支原体（mycoplaa pneumoniae，MP）介于细菌与病毒之间，属于最小原核细胞型微生物，无细胞壁，可引起致命的呼吸道感染，甚至引起肺外严重并发症，在婴幼儿及青少年间呈区域性流行。近年来MP感染的发病率逐年上升，对儿童的健康造成很大的危害。MP发病初期症状不明显，很难与病毒性和细菌性呼吸道感染相鉴别，有时伴其他并发症更难以确诊本病。MP培养生长缓慢，通常需要21 d，甚至更久，而MP抗体一般在发病后1周出现，导致诊疗延误，但是其用药治疗与细菌性、病毒性感染均不同。因此，快速准确诊断MP在临床上显得特别重要。2009年1月～2011年12月笔者采用MP快速鉴定培养基[3-4]对1 620例急性呼吸道感染患者咽拭子进行培养检测，现将分析结果报道如下：
[关键词] 肺炎支原体；快速鉴定；培养基
[] C[文章编号] 1673-7210（2012）08（c）-0156-02
Results and analysis of medium for rapid identification of mycoplaa pneumoniae culture
PENG Ping XU Xiaodu WANG Guangbin YE Dan ZHANG Ting WU Bo
The First People′s Hospital of Longquanyi District in Chengdu City, Sichuan Province, Chengdu 610100, China
[Abstract] Objective To investigate the rapid mycoplaa pneumoniae (MP) identification of culture medium in the diagnosis of early infection. Methods 1 620 cases suffered from respiratory tract infection in our hospital from January 2009 to December 2011 were examined with rapid identification of MP medium. Results 656 cases of the throat swab specimens were confirmed MP positive, with a total positive rate of 40.49%; the positive rate was 40.80% in male and 40.13% in female, the difference between male and female was not statistically significant (P > 0.05); the positive rate was 45.95% of ≤ 2 year children and 31.41% of 3-14 year old children, the difference was statistically significant (P > 0.05); the positive rate in the season of summer and autumn was lower than that in spring, the difference was all statistically significant (all P > 0.05). Conclusion The rapid identification of medium detection of mycoplaa pneumoniae infection has a higher overall detection rate and a accurate, simple, rapid result, it is a laboratory diagnostic test of choice for MP infection.
[Key words] Mycoplaa pneumoniae; Rapid identification; Medium
肺炎支原体（mycoplaa pneumoniae，MP）介于细菌与病毒之间，属于最小原核细胞型微生物，无细胞壁，可引起致命的呼吸道感染，甚至引起肺外严重并发症，在婴幼儿及青少年间呈区域性流行。近年来MP感染的发病率逐年上升，对儿童的健康造成很大的危害。MP发病初期症状不明显，很难与病毒性和细菌性呼吸道感染相鉴别，有时伴其他并发症更难以确诊本病。MP培养生长缓慢，通常需要21 d，甚至更久，而MP抗体一般在发病后1周出现，导致诊疗延误，但是其用药治疗与细菌性、病毒性感染均不同。因此，快速准确诊断MP在临床上显得特别重要。2009年1月～2011年12月笔者采用MP快速鉴定培养基[3-4]对1 620例急性呼吸道感染患者咽拭子进行培养检测，现将分析结果报道如下：
1 资料与方法
源于：论文的格式要求www.udooo.com
1.1 一般资料
2009年1月～2011年12月在我院门诊及住院的呼吸道感染患者咽拭子标本1 620例，其中男820例，女800例。≤2岁者1 012例，3～14岁者608例。1.2 方法
本资料采用MP快速鉴定培养基（陕西百盛园生物科技信息有限公司）进行检测，严格按照使用说明书进行操作。①分装冷冻培养基常温复温，使其恢复到液体状态。②无菌的咽拭子在被检患儿咽喉部捻转数次采集分泌物并取出。③无菌状态下打开培养基瓶盖，把棉拭子置于瓶内搅动数次，将拭子在瓶内壁挤压，将尽量多的液体挤到培养基中，除去棉拭子，封闭瓶盖，置于37℃恒温箱中培养24 h。源于：论文参考文献标准格式www.udooo.com